NFκB p65 antibody (pAb)

RRID: AB_2793217
Aliases: RELA, p65, NFKB3
Catalog No: 39283 Format: 200 µl ¥5,670 Add to Cart
Catalog No: 39284 Format: 10 µl ¥1,430 Add to Cart

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
65 kDa
Reactivity:
Human

Applications

CUT&RUN Validated Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 1:500 - 1:2,000 dilution
CUT&RUN: 1 µl per 50 µl reaction

Immunogen

This NFkB p65 antibody was raised against a peptide immunogen corresponding to N-terminal p65.

Buffer

Immunoaffinity purified antibody formulated in 30% glycerol containing 0.035% sodium azide.

 
NFκB p65 antibody (pAb) tested by Western blot.

NFκB p65 pAb tested by Western blot.
NFκB p65 detection by Western blot. The analysis was performed using 20 µg HeLa nuclear extract and NFκB p65 pAb at a 1:2,000 dilution.

NFκB p65 antibody (rAb) tested by CUT&RUN

NFκB p65 antibody (pAb) tested by CUT&RUN
CUT&RUN was performed using 500,000 Hela nuclei and sequenced using 38 base-pair, paired-end reads on the Illumina NovaSeq. Data was collected from 16 million reads, and NFkB data is shown for Chromosome 19.

Background

NFκB p65 (Nuclear factor NF-kappa-B p65 subunit also known as NFκB p65 or RelA) is a member of the Rel family of transcription factors that are involved in processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. The Rel family includes c-Rel, RelA (NFκB p65, NFκB3) and RelB (I-Rel). Rel proteins form dimers with the NFκB p50 (proteolytically processed from p105/NFκB1) and p52 (proteolytically processed from p100/NFκB2) members of the NFκB family that bind DNA and activate transcription. The NFκB p65/p50 heterodimer is the most abundant form of NFκB.

NFκB signaling is negatively regulated by the sequestration of the NFκB complex in the cytoplasm by its association with the IκB family of inhibitory proteins. The IκB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFκB. The IKK complex is composed of two catalytic subunits (IKKα and IKKβ) and a regulatory subunit (IKKγ). Upon its activation, the IKK complex phosphorylates IκB proteins (IκBα, IκBα phospho Ser32, 36), which marks them for ubiquitination and degradation. This enables the NFκB complex to translocate to the nucleus where it regulates transcription by binding to DNA.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for NFκB p65 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting