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IκBα phospho Ser32,36 antibody (mAb)
| RRID: AB_2793427 |
| Clone: 39A1431 |
| Aliases: NFKBIA, MAD-3, NFKBI |
| Catalog No: 40904 | Format: 100 µg | ¥3,630 | Add to Cart |
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Applications
Application Notes
Applications Validated by Active Motif:
WB: 1 - 2 µg/ml dilution
Immunogen
This IκBα phospho Ser32,36 antibody was raised against a synthetic peptide containing phosphorylated serines at amino acid residues 32 and 36 of human IκBα.
Buffer
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
IκBα phospho Ser32,36 mAb tested by Western blot.
Jurkat cells were treated for 30 minutes with 100 µg/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal), a calpain and proteasome inhibitor that prevents IκBα dephosphorylation, followed by incubation with (lanes 2 & 4) or without 1 nM TNF-α (1 & 3). The membranes were blotted with the anti-phospho-IκBα (lanes 1 & 2) or anti-IκBα, which recognizes both phosphorylated and non-phosphorylated forms of IκBα. The data shows that IκBα phospho Ser32,36 mAb detects specifically the phosphorylated forms of IκBα.
Background
IκBα phospho Ser32,36 – NFκB (NFκB p50 & NFκB p65) signaling is controlled to a large extent by the sequestration of the NFκB complex in the cytoplasm by its association with one of the IκB family of proteins. IκBα is phosphorylated at Ser32 and Ser36 by the IκB Kinase (IKK) complex, resulting in the degradation of IκB and the nuclear translocation of NFκB.
Positive Control
Extracts from Jurkat cells treated for 30 minutes with 100 µg/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal), followed by incubation with or without 1 nM TNF-α cell extract can be used as a positive control for IκBα phospho Ser32, 36 antibody.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Store at 4°C for short term. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
