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CUT&Tag-IT® Express Assay Kit

Less variable and higher-throughput genome-wide profiling of histone marks

 

CUT&Tag-IT® Express Assay Kit Overview

CUT&Tag-IT Express Assay Kit
 

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Discover a new standard in CUT&Tag epigenomic profiling —our improved CUT&Tag-IT® Express Assay Kit. Engineered to deliver unparalleled sensitivity and reproducibility, this kit streamlines the CUT&Tag assay workflow by integrating simultaneous tagmentation and adapter incorporation into a single, efficient 0.2 mL reaction, utilizing silica beads for DNA purification for a faster workflow.

Designed for low-input samples and optimized for minimal background noise, our assay kit empowers you to generate robust, next-generation sequencing–ready libraries with ease, accelerating your epigenomic discoveries and reducing costs. Whether you’re working with rare cell populations or complex tissue samples, experience exceptional performance and simplified operation that redefines precision in chromatin mapping.

CUT&Tag-IT Express Assay Kit Advantages:

  • More consistent results
  • Simplified workflow
  • Higher throughput sample processing
  • Includes mouse and rabbit secondary antibodies

Experience the power of precise, consistent epigenetic mapping that outperforms traditional methods. Our optimized CUT&Tag not only simplifies the process but also delivers superior clarity, empowering you to confidently explore gene regulation and chromatin architecture—even with minimal input material. Transform your research with the cutting-edge, less variable CUT&Tag-IT Express technology and drive your discoveries faster.

CUT&Tag-IT Express Assay Kit is designed to work with fresh or frozen cell samples, and will work with tissue samples processed with our Tissue Prep for NGS Assays kit.

Explore how our Improved CUT&Tag Assay Kit transforms your research into breakthrough insights.

 

How does CUT&Tag-IT Express Assay Kit compare with the original CUT&Tag-IT Assay Kit?

Kit Assay Format DNA Purification Method Tn5 Release
Original CUT&Tag-IT Assay Kit (53160, 53165) 1.5 mL tubes Columns Separate steps
CUT&Tag-IT Express Assay Kit 0.2 mL tubes Silica beads Combined release step


 

CUT&Tag-IT® Express Assay Kit Contents

Expand to View Kit Content

Component Storage temp
5% Digitonin -20°C
Concanavalin A Beads 4°C
CUT&Tag-IT™ Assembled pA-Tn5 Transposomes -20°C
Tagmentation Buffer 4°C or -20°C
1X Binding Buffer 4°C
1X Wash Buffer 4°C
Dig-Wash Buffer 4°C
Antibody Buffer 4°C
Dig-300 Buffer 4°C
Alpaca Anti-Rabbit IgG (H+L) -20°C
Rabbit Anti-Mouse Antibody -20°C
Protease Inhibitor Cocktail -20°C
Glycogen -20°C
10 µg/µL Proteinase K -20°C
Silica Beads 4°C
DNA Purification Binding Buffer RT
DNA Purification Wash Buffer RT
DNA Purification Elution Buffer RT
3M Sodium Acetate RT
Q5 DNA Polymerase Master Mix (2U/µL) -20°C
Tn5 Release Solution -20°C
i5 Indexed Primer 1 -20°C
i5 Indexed Primer 2 -20°C
i5 Indexed Primer 3 -20°C
i5 Indexed Primer 4 -20°C
i7 Indexed Primer 1 -20°C
i7 Indexed Primer 2 -20°C
i7 Indexed Primer 3 -20°C
i7 Indexed Primer 4 -20°C
SPRI Beads 4°C


 

CUT&Tag-IT® Express Assay Kit Data

Improved performance
(Click image to enlarge)

Figure 1. CUT&Tag-IT Express Assay Kit has improved performance over original CUT&Tag-IT Assay Kit
250,000 K562 cells were assayed with CUT&Tag-IT Express and CUT&Tag-IT Assay Kits with H3K4me3, H3K27me3, and H3K27Ac. CUT&Tag-IT Express delivers higher peak numbers, higher FRiP scores, and lower duplication rates than the original CUT&Tag-IT Assay Kit.

CUT&Tag-IT Express Assay Kit Performance
(Click image to enlarge)

Figure 2. CUT&Tag-IT Express Assay Kit Performance Compared with Direct PCR Method.
250,000 K562 cells were assayed with CUT&Tag-IT Express for H3K27me3 and purified with the Silica Beads in the kit or taken through direct PCR without purification. FRiP scores plotted in copper alongside Peak numbers in purple. Greater peak numbers and higher FRiP scores resulted from CUT&Tag-IT Express Assay Kit than going directly to PCR without purification.

CUT&Tag-IT Express Assay Kit Silica Bead Purification
(Click image to enlarge)

Figure 3. CUT&Tag-IT Express Assay Kit Silica Bead Purification is Improved Performance Over Original CUT&Tag-IT Assay Kit DNA Purification Columns.
250,000 K562 cells were assayed in duplicate with CUT&Tag-IT Express or CUT&Tag-IT Assay Kits for H3K27me3. TapeStation results are shown, and the yield of library generated is plotted for the respective sample lanes. The column purification method of the original CUT&Tag-IT Assay kit yielded 5,280 and 4,120 pmol/L libraries, and the CUT&Tag-IT Express Assay Kit Silica Beads yielded 13,300 and 16,400 pmol/L. CUT&Tag-IT Express consistently yields more library than the original CUT&Tag-IT Assay Kit.

 

CUT&Tag-IT® Express Assay Kit Documents

 

 

You might also be interested in:

US Pat. No. 10,689,643, EP Pat. No. 2999784 and related patents and applications

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In the absence of an express written agreement to the contrary, all products are sold and services deliverables are provided by Active Motif for (a) internal in vitro research purposes only and may not be used for services or any other commercial purpose (b) the exclusive use of the original purchaser, and are not to be resold. You agree not to reverse engineer or otherwise attempt to discover the structure or composition of products or services deliverables unless we otherwise agree in writing.

 
Name Format Cat No. Price  
CUT&Tag-IT® Express Assay Kit 16 rxns 53175 ¥18,330 Add to Cart
CUT&Tag-IT® Express Assay Kit Manual