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Chromeo™ Dyes – Chromeo 488, Chromeo 494, Chromeo 546 and Chromeo 642 – exhibit superior fluorescent properties, broad Stokes shifts, stability towards photobleaching and pH, and low cell toxicity. In addition, they are compatible with most excitation sources including diode lasers, LEDs, tungsten lamps and xenon arc lamps. For use in bio-orthogonal labeling, Chromeo Dyes are offered as click-reactive azides or alkynes.
Figure 1: Click-labeling with Chromeo Dye azides.
Chromeo™ Azide and Alkyne advantages
- High fluorescent intensity
- Photostability – enables multiple exposures and increased exposure time
- pH insensitive
- Low background
- Ready to use in click-reactions
In addition to click chemistry reagents, Chromeo Dyes are offered in a variety of formats like amine reactive NHS-Esters, which have been shown to label amines in peptides, proteins or amino modified DNA.
Figure 2: Chromeo 546 staining in HeLa cells.
HeLa cells were stained with alpha Tubulin mouse mAb (Clone 5-B-1-2) and Chromeo 546 Goat anti-mouse IgG.
| Dye | Absorption | Emission | Spectra | ε L/(mol-cm) | Stokes shift |
|---|---|---|---|---|---|
| Chromeo™ 488 | 488 nm | 517 nm |  |
73,000 | 29 nm |
| Chromeo™ 494 | 494 nm | 628 nm |  |
55,000 | 134 nm |
| Chromeo™ 546 | 545 nm | 561 nm |  |
98,800 | 16 nm |
| Chromeo™ 642 | 642 nm | 660 nm |  |
180,000 | 18 nm |
| Table 1: Properties of Chromeo™ Dyes. | |||||
To receive more detailed information and application data for individual Chromeo Dyes, simply click on the name of the Chromeo Dye of your choice in the table above. To see a larger spectra image, click on the spectrum of your choice.
