Cart
Products
Recombinant UBE2C protein
| Aliases: Ubiquitin conjugating enzyme E2 C, dj447F3.2, UBCH10 |
| Catalog No: 82010 | Format: 100 µg | ¥3,840 | Add to Cart |
| Catalog No: 82610 | Format: 1 mg | ¥23,600 | Add to Cart |
Request a quote for a bulk order Request Quote
Expressed In: E. coli Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
UBE2C (Ubiquitin-conjugating enzyme E2 C) also known as dJ447F3.2 and UBCH10. UBE2C is a member of the E2 ubiquitin-conjugating enzyme family which is required for post-replicative DNA damage repair and plays an important role in various cellular processes. UBE2C can accept ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes ‘Lys-11’- and ‘Lys-48’-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating ‘Lys-11’-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for this product is shown.
Protein Details
Recombinant UBE2C protein that includes full length of human UBE2C protein (accession number NP_008950.1) was expressed in E. coli and contains an N-terminal His tag with a molecular weight of 21.82 kDa. The purity of the protein is ≥ 90% by SDS-PAGE.
References
Human
Recombinant UBE2C protein gel.
UBE2C protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue.
MW: 21.82 kDa
Purity: >90%
AMP-Glo assay for UBE2C activity
7.9 μM ubiquitin, 63 nM UBA1 and 25 μM ATP were incubated with different concentrations of UBE2C in 10 μl reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37℃ for 1 hour. 10 μl of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20 μl of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.
