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Recombinant MAP3K8 (30-397) / COT protein
| Aliases: (Mitogen-Activated Protein Kinase Kinase Kinase 8 |
| Catalog No: 81380 | Format: 20 µg | ¥3,840 | Add to Cart |
| Catalog No: 81680 | Format: 1 mg | ¥33,200 | Add to Cart |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
MAP3K8 (Mitogen-Activated Protein Kinase Kinase Kinase 8) is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. It also involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells and involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro>, MAP3K8 activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner and leading to up-regulation of IL8 and CCL4 and transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages. Thus MAP3K8 may play a role in the regulation of immunoglobulin production and may also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, MAP3K8 activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF.
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Protein Details
Recombinant MAP3K8 / COT (30-397) protein that includes amino acids 30-397 of human MAP3K8 protein (accession number NP_001231063.1) was expressed in a baculovirus expression system, and contains an N-terminal FLAG tag. The molecular weight of the protein is 42.8 kDa.
References
Human
Recombinant MAP3K8 / COT (30-397) protein
10% SDS-PAGE with Coomassie staining
MW: 42.8 kDa
Purity: >50%
HTRF assay for MAP3K8 / COT (30-397) activity
1 µM STK S3 substrate was incubated with different concentrations of MAP3K8 / COT (30-397) protein in a 10 µl reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 µM ATP for 1 hour. Then 10µμl detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of arrival.
