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Recombinant RPS6KA3/MAPKAPK1B protein
| Aliases: RPS6KA3, ISPK1, MAPKAPK1B, RSK2, Ribosomal protein S6 kinase alpha-3, 90kD ribosomal protein S6 kinase 3, Insulin-stimulated protein kinase 1, MAP kinase-activated protein kinase 1b, Ribosomal S6 kinase 2, pp90RSK2 |
| Catalog No: 81363 | Format: 20 µg | ¥3,840 | Add to Cart |
| Catalog No: 81663 | Format: 1 mg | ¥33,200 | Add to Cart |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
RPS6KA3 also known as MAPKAPK1B is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signaling pathway. It acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RPS6KA3 also regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1.Diseases associated with RPS6KA3 include Coffin-Lowry Syndrome and Symptomatic Form Of Coffin-Lowry Syndrome In Female Carriers. Among its related pathways are Cellular Senescence (REACTOME) and Activated TLR4 signaling.
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Protein Details
Recombinant RPS6KA3 / MAPKAPK1B protein was expressed in a baculovirus expression system as the full length protein (accession number NP_004577.1) with a N-terminal FLAG tag. The molecular weight of the protein is 84.7 kDa.
References
Human
Recombinant RPS6KA3 / MAPKAPK1B protein
10% SDS-PAGE with Coomassie blue staining
MW: 84.7 kDa
Purity: >95%
HTRF assay for RPS6KA3 / MAPKAPK1B activity
1 µM STK S1 substrate was incubated with different concentrations of RPS6KA3 / MAPKAPK1B protein in a 10 µl reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100 µM ATP for 1 hour. Then 10 µl detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of arrival.
