Recombinant EEF1G protein

Aliases: Eukaryotic Translation Elongation Factor 1 Gamma
Catalog No: 81222 Format: 100 µg ¥3,920 Add to Cart
Catalog No: 81922 Format: 1 mg ¥25,600 Add to Cart

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Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

EEF1G (Eukaryotic Translation Elongation Factor 1 Gamma), also called EF1G or GIG35, is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases. EEF1G probably plays a role in anchoring the complex to other cellular components.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product.  All available data this product is shown. 

Protein Details

Recombinant EEF1G protein was expressed in E.coli as the full length protein (accession number NP_001395.1) with an N-terminal 6×His tag. The molecular weight of the protein is 53.7 kDa.

 
Recombinant EEF1G protein gel

Recombinant EEF1G protein gel
12.5% SDS-PAGE gel
MW: 53.7 kDa
Purity: >92%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.