Recombinant EIF4A1 protein

Aliases: Eukaryotic Translation Initiation Factor 4A1, DDX2A
Catalog No: 81216 Format: 50 µg ¥3,920 Add to Cart
Catalog No: 81916 Format: 1 mg ¥25,600 Add to Cart

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Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

EIF4A1 (Eukaryotic Translation Initiation Factor 4A, Isoform 1), also called as EIF4A or DDX2A, is a ATP dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition. It is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5’-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. This protein is involved in the binding of mRNA to 40S subunit of ribosome,ubiquitous with high expression in skeletal muscle and ovary. The helicase activity of EIF4A1 and function in translation are inhibited by interaction with PDCD4.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product.  All available data this product is shown. 

Protein Details

Recombinant EIF4A1 protein was expressed in E.coli as the full length protein (accession number NP_001407.1) with an N-terminal 6xHis Tag. The molecular weight of the protein is 49.7 kDa.

 
Recombinant EIF4A1 protein gel

Recombinant EIF4A1 protein gel
10% SDS-PAGE gel with Coomassie staining
MW: 49.7 kDa
Purity: >90%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.