HNF-3β / FOXA2 antibody (pAb)

RRID: AB_2793357
Aliases: TCF3B
Catalog No: 39827 Format: 100 µl ¥5,670 Add to Cart
Catalog No: 39828 Format: 10 µl ¥1,430 Add to Cart

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
50 kDa
Reactivity:
Human, Rat

Applications

Western Blot Validated Immunohistochemistry Validated

Application Notes

Applications Validated by Active Motif:
WB: 1:2,000 - 1:5,000 dilution

Immunogen

This HNF-3β / FOXA2 antibody was raised against a recombinant protein corresponding to amino acids 7-86 of rat HNF-3β /FOXA2.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 
HNF-3β / FOXA2 antibody (pAb) tested by Western blot.

HNF-3β / FOXA2 antibody (pAb) tested by Western blot.
Extract of HeLa cells expressing HNF-3β / FOXA2 (50 µg per lane) probed with HNF-3β / FOXA2 antibody (pAb) (1:5,000 dilution).

Background

HNF-3β (Hepatocyte nuclear factor 3 beta, forkhead box A2, FoxA2) is a homeodomain containing transcription factor and is a member of the Hepatocyte Nuclear Factor family (HNF-1α, HNF-1β, HNF-3α, HNF-3β, HNF-3γ, HNF-4α, HNF-4γ, HNF-6α and HNF-6β), whose members regulate the transcription of numerous hepatocyte genes in adult liver. HNF-3 members bind to DNA as monomers using a winged helix DNA-binding domain. HNF-3α and HNF-3γ have also been shown to be involved in gastrulation events such as body axis formation.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for HNF-3β / FOXA2 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting