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Ikaros antibody (mAb)
| RRID: AB_2614961 |
| Clone: 2A9 |
| Aliases: IKZF1, LyF-1, hlk-1, Ikaros, Zfpn1a1, Znfn1a1 |
| Catalog No: 39355 | Format: 200 µg | ¥5,830 | Add to Cart |
| Catalog No: 39356 | Format: 10 µg | ¥1,430 | Add to Cart |
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Applications
Application Notes
Applications Validated by Active Motif:
ChIP: 4 µg per ChIP
ChIP-Seq: 4 µg each
WB: 0.5 - 2 µg/ml dilution
ChIP-Seq validation was performed by Active Motif's Epigenetics Services; the complete data set is available in the UCSC Genome Browser by clicking here.
Immunogen
This Ikaros antibody was raised against a recombinant protein corresponding to the N-terminal half of mouse Ikaros.
Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Ikaros antibody (mAb) (Clone 2A9) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from mouse spleen and 4 µg of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify Ikaros binding sites. The image shows binding across a region of chromosome 9. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.
Ikaros antibody (mAb) (Clone 2A9) tested by Western blot.
Detection of Ikaros by Western blot analysis. Ikaros detected in 20 µg mouse spleen whole-cell extract using Ikaros mAb (Clone 2A9) at a 2 µg/ml dilution.
Background
Ikaros is a transcription factor that plays a critical role in the control of lymphohematopoiesis and immune regulation. All Ikaros isoforms share a common C-terminal domain that contains a transcription activation motif and two zinc finger motifs required for hetero- and homo-dimerization among the Ikaros isoforms, and for interactions with other proteins. Ikaros is abundantly expressed in pituitary mammosomatotrophs, where it deacetylates Histone H3 sites on the proximal growth hormone (GH) promoter to silence gene expression.
Positive Control
Whole-cell extracts from mouse spleen can be used as a positive control for Ikaros antibody (Clone 2A9).
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Ikaros antibody (mAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
