AbFlex® Bcl6 antibody (rAb)

RRID: AB_2793833
Aliases: B-Cell CLL/Lymphoma 6, BCL5, LAZ3, BCL6A, ZNF51, ZBTB27
Catalog No: 91287 Format: 100 µg ¥4,400 Add to Cart
Catalog No: 91288 Format: 10 µg ¥990 Add to Cart

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Antibody Type:
Recombinant
Isotype:
IgG2a
Purification:
Protein A Chromatography
Host:
Mouse
Molecular Weight:
80 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 0.5 – 2 µg/ml

AbFlex® recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61193.

Immunogen

This antibody was raised against full length human Bcl6 (B-cell lymphoma 6 protein) by genetic immunization. (Garcia et. al., 2006. Genetic Immunization: A New Monoclonal Antibody for the Detection of BCL-6 Protein in Paraffin Sections. J. Histochemistry & Cytochemistry, vol. 54(1): 31-38).

Buffer

140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, and 30% glycerol. Sodium azide is highly toxic.

 
AbFlex<sup>®</sup> Bcl6 antibody (rAb) tested by Western blot.

AbFlex® Bcl6 antibody (rAb) tested by Western blot.
20 µg of U87 cell nuclear extract was run on SDS-PAGE and probed with AbFlex Histone Bcl6 antibody at 0.5 µg/ml.

Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex® Bcl6 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

Bcl6 (B-cell lymphoma 6 protein, Bcl5, LAZ3, ZBTB27, ZNF51) is sequence-specific transcriptional repressor that is required for the formation of lymph node germinal centers (GC). Bcl6 is a member of the BTB-POZ family of transcriptional regulators and contains six Kruppel-type zinc fingers. The POZ domain in the amino terminus of Bcl6 is involved in the recruitment of corepressor proteins including NCoR, BCoR, SMRT, Mi-2/NURD and several HDACs. Bcl6 promotes proliferative expansion of B-cells in the germinal center through repression of genes regulating cell cycle and differentiation. Bcl6 also suppresses the DNA damage response, to prevent class-switch recombination being repaired. Unregulated expression of Bcl6 is involved in the development of many B-cell lymphomas.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting