Recombinant Histone H3/H4 tetramer

Catalog No: 81169 Format: 100 µg ¥4,040 Add to Cart
Catalog No: 81869 Format: 1 mg ¥29,600 Add to Cart

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Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

Histone H3 and H4 are the core components of the nucleosome. H3 and H4 assemble H3/H4 tetramer, and H3/K4 tetramer combines with two H2A/H2B dimers to form histone octamer. The histone octamer is wrapped around by 146 bp DNA to assemble a nucleosome, which is the basic structural unit of chromatin in vivo.

Application Notes

Recombinant Histone H3/H4 Tetramer is suitable for use as substrates in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Protein Details

Recombinant histone H3/K4 Tetramer consists of full length human histones H3 (accession number NP_003520.1) and full length human histone H4 (accession number NP_003539.1). Histone H3 and H4 were expressed in E. coli cells. Recombinant histone H3/H4 Tetramer was assembled by dialysis and purified by gel filtration. The molecular weight of the Histone H3/H4 Tetramer is 53 kDa.

 
Recombinant Histone H3/H4 Tetramer

Recombinant Histone H3/H4 Tetramer
13% SDS-PAGE Coomassie staining

MW:
H3: 15.3 kDa
H4: 11.2 kDa

Purity: ≥ 90%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.