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Recombinant EPHA5 (620-1037) protein
| Aliases: Ephrin type-A receptor 5, EPHA5, CEK7, EHK1, Hek7, TYRO4 |
| Catalog No: 81495 | Format: 20 µg | ¥4,160 | Add to Cart |
| Catalog No: 81595 | Format: 1 mg | ¥33,200 | Add to Cart |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
EPHA5 (Ephrin type-A receptor 5) is a receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino-hippocampal and hippocamposeptal pathways. Together with EFNA5 also plays a role in synaptic plasticity in adult brain through regulation of synaptogenesis. In addition to its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion.
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Protein Details
Recombinant EPHA5 (620-1037) protein that includes amino acids 620-1037of human EPHA5 protein (accession number NP_004430.4) was expressed in a baculovirus expression system, and contains an N-terminal FLAG tag. The molecular weight of the protein is 48.41 kDa.
References
Human
Recombinant EPHA5 (620-1037) protein
10% SDS-PAGE Coomassie staining
MW: 48.41 kDa
Purity: ≥ 85%
HTRF assay for EPHA5 (620-1037) activity
1 µM TK substrate was incubated with different concentrations of EPHA5 (620-1037) protein in a 10 µl reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2,1mM MnCl2, 1 mM DTT, 5nM SEB and 100 µM ATP for 1 hour. Then 10 µl detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.
HTRF assay for EPHA5 (620-1037) activity
1 µM TK substrate was incubated with different concentrations of EPHA5 (620-1037) protein in a 10 µl reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2,1mM MnCl2, 1 mM DTT, 5nM SEB and 100 µM ATP for 1 hour. Then 10 µl detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
