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Recombinant FYN (2-537) protein
| Aliases: FYN Proto-Oncogene, Src Family Tyrosine Kinase) |
| Catalog No: 81368 | Format: 20 µg | ¥3,840 | Add to Cart |
| Catalog No: 81668 | Format: 1 mg | ¥33,200 | Add to Cart |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
FYN (FYN Proto-Oncogene, Src Family Tyrosine Kinase) is a non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/ FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. FYN involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin) and regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubuleassociated proteins MAP2 and MAPT. It also promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Disieases associated with FYN include Hepatitis E and Autoimmune Cardiomyopathy
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Protein Details
Recombinant FYN (2-537) protein that includes amino acids 2-537 of human FYN protein (accession number NP_001357458.1) was expressed in a baculovirus expression system, and contains an N-terminal FLAG tag. The molecular weight of the protein is 61.6 kDa.
References
Human
Recombinant FYN (2-537) protein
10% SDS-PAGE gel with Coomassie blue staining
MW: 61.6 kDa
Purity: >95%
HTRF assay for FYN (2-537) activity
1 µM TK substrate was incubated with different concentrations of FYN (2-537) protein in a 10 µl reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 µM ATP for 1 hour. Then 10 µl detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection
HTRF assay for FYN (2-537) activity
1 µM TK substrate was incubated with different concentrations of FYN (2-537) protein in a 10 µl reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 µM ATP for 1 hour. Then 10 µl detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of arrival.
