Recombinant beta-Glucosyltransferase protein

Aliases: b-GT, beta-GT
Catalog No: 81249 Format: 100 µg ¥3,760 Add to Cart
Catalog No: 81949 Format: 1 mg ¥25,600 Add to Cart

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Expressed In: E. coli Protein Species: Escherichia virus T4

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

Beta-Glucosyltransferase, also called as β-Glucosyltransferase or BGT, is a DNA-modifying enzyme. It is encoded by a number of bacteriophages belonging to the T-even group. It catalyses the transfer of glucose (Glc) from uridine diphosphoglucose (UDP-Glc) to 5-hydroxymethylcytosine (5-HMC) in double-stranded DNA. BGT shows little DNA sequence specificity but modify only 5-HMC bases. The glucosylation protects the infecting viral DNA from host nucleases. In addition to its protective role, the glucosylation has also been implicated in the control of phage-specific gene expression by influencing transcription in vivo and in vitro. The reactions is: UDP-Glc + 5-HMC-DNA → β-glucosyl- HMC-DNA + UDP.

Application Notes

Recombinant beta-Glucosyltransferase protein is useful for the study of glucosyltransferase assays in vitro.

Protein Details

Beta-Glucosyltransferase was expressed in E. coli cells as the full length protein (accession number NP_049658.1) with an N-terminal 6×His tag. The molecular weight of beta-Glucosyltransferase protein is 42.8 kDa.

 
ecombinant beta-Glucosyltransferase protein

Recombinant beta-Glucosyltransferase protein
10% SDS-PAGE gel with Coomassie Blue staining
MW: 42.8 kDa
Purity: ≥90%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.