WDR4 antibody (pAb)

RRID: AB_3216374
Catalog No: 65113 Format: 100 µg ¥5,670 Add to Cart
Catalog No: 65114 Format: 10 µg ¥1,430 Add to Cart
Catalog No: 69113 Format: 50 µg ¥3,250 Add to Cart

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Protein A Chromatography
Host:
Rabbit
Molecular Weight:
45 kDa

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 0.5 - 2 µg/ml

*Note: For optimal results, we recommend the addition of 0.05% Tween-20 to all blocking solutions to reduce background in Western blot.

Immunogen

This antibody was raised against a human peptide containing WD repeat domain 4.

Buffer

Purified in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 
WDR4 antibody (pAb) tested by Western blot.

WDR4 antibody (pAb) tested by Western blot
20 µg of HeLa whole cell extract was run on SDS-PAGE and probed with antibody at a dilution of 2µg/mL.

Background

WDR4 (WD Repeat-Containing Protein 4), also known as TRM82, is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. WDR4 is the non-catalytic subunit of N7-methylguanosine (m7G). WRD4 and METTL1 form a complex required for tRNA (guanine(46)-N(7))-methyltransferase activity. This complex catalyzes tRNA m7G methylation to enhance translation efficiency.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting