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N6-Methyladenosine (m6A) antibody (pAb)
| RRID: AB_2793658 |
| Catalog No: 61495 | Format: 100 µg | ¥5,670 | Add to Cart |
| Catalog No: 61496 | Format: 10 µg | ¥1,430 | Add to Cart |
| Catalog No: 61995 | Format: 50 µg | ¥3,250 | Add to Cart |
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Applications
Application Notes
Applications Validated by Active Motif:
DB: 1 - 2 µg/ml dilution
For N6-Methyladenosine (m6A), we also offer AbFlex® N6-Methyladenosine (m6A) Recombinant Antibody (rAb). For details, see Catalog No. 91261.
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
DB
Liquid chromatography / Mass Spec
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This antibody was raised against 6-Methyladenosine conjugated to KLH.
Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
N6-Methyladenosine (m6A) antibody (pAb) tested by RNA dot blot analysis.
3.3ng of a 22 nt RNA containing four repeats of the GAC consensus for N6-methyladenosine within its sequence was spotted onto a membrane and probed with N6-Methyladenosine antibody (1 µg/ml). The sequence contained various adenosine modifications at all four positions as indicated.
Lane 1: Adenosine.
Lane 2: N6-methyladenosine.
Lane 3: 1-methyladenosine.
Lane 4: 2’-O-methyladenosine.
Lane 5: inosine
N6-Methyladenosine (m6A) antibody (pAb) tested by DNA dot blot analysis.
Single-stranded DNA oligonucleotides (amount of oligo in pmoles listed on the left side of the blot) were spotted on to a positively charged nylon membrane and blotted with N-6-methyladenosine antibody (1 µg/ml dilution).
Lane 1: DNA containing a single 6-methyladenosine.
Lane 2: DNA containing unmethylated adenosine.
Lane 3: DNA containing a single 1-methyladenosine.
Background
N6-Methyladenosine (m6A) ) is an RNA modification on the N-6 position of adenosine. This modification has been found to be abundant in the 3’ UTR and stop codons of mammalian mRNA. m6A is associated with miRNA binding sites suggesting a potential role in epigenetic gene regulation. FTO and ALKBH are demethylases for 6-methyladenosine while a multiprotein complex that includes METTL3 functions as the methyltransferase. Recent findings revealed that m6A is also present on metazoan DNA, suggesting a genuine epigenetic role for this modification in the context of DNA as well.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
