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OGA/O-GlcNAcase antibody (pAb)
| RRID: AB_2793630 |
| Aliases: MGEA5, OGA, MEA5, NCOAT |
| Catalog No: 61425 | Format: 100 µl | ¥5,670 | Add to Cart |
| Catalog No: 61426 | Format: 10 µl | ¥1,430 | Add to Cart |
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Applications
Application Notes
Applications Validated by Active Motif:
IP: 10 µl per IP
WB: 1:500 - 1:2,000 dilution
The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.
Immunogen
This antibody was raised against a peptide within the N-terminal region of human OGA/ O-GlcNAcase.
Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.
OGA/O-GlcNAcase antibody (pAb) tested by Immunoprecipitation.
10 µl of OGA/O-GlcNAcase antibody was used to immunoprecipitate OGA/O-GlcNAcase from 400 µg of HeLa whole cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the OGA/O-GlcNAcase antibody at a dilution of 1:1,000.
OGA/O-GlcNAcase pAb tested by Western blot.
OGA/O-GlcNAcase detection by Western blot. The analysis was performed using 20 µg HeLa whole cell extract and OGA/O-GlcNAcase at a 1:1,000 dilution.
Background
OGA / O GlcNAcase (O-linked N-acetylglucosamine (GlcNAc) -ase) is a glycosidase that removes O-linked N-acetylglucosamine (O-GlcNAc) modifications from serine and threonine residues on nuclear and cytoplasmic proteins. This dynamic modification is catalyzed by OGT and plays a role in homeostatic mechanisms and influences gene expression.
Positive Control
Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for OGA/O-GlcNAcase antibody.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
