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SMARCC1 / BAF155 antibody (pAb)
| RRID: AB_2793624 |
| Aliases: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1 / BRG1-associated factor 155, Rsc8, SWI3, BAF155, CRACC1 |
| Catalog No: 61409 | Format: 100 µl | ¥5,670 | Add to Cart |
| Catalog No: 61410 | Format: 10 µl | ¥1,430 | Add to Cart |
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Applications
Application Notes
Applications Validated by Active Motif:
ICC/IF: 1:2,000 dilution
WB*: 1:500 - 1:1,000 dilution
The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.
*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Immunogen
This antibody was raised against a peptide within the N-terminal region of human SMARCC1 / BAF155.
Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.
SMARCC1 / BAF155 antibody (pAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with SMARCC1 / BAF155 antibody at a 1:2,000 dilution.
SMARCC1 / BAF155 antibody (pAb) tested by Western blot.
K-562 nuclear extract (30 µg) probed with SMARCC1 / BAF155 antibody at a dilution of 1:500.
Background
SMARCC1 / BAF155 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1 / BRG1-associated factor 155) is involved in transcriptional activation and repression of select genes by chromatin remodeling. Component of multiple SWI/SNF chromatin remodeling complexes including Swi/Snf-A (BAF), Swi/Snf-B (PBAF), Brm, Brg1(I), WINAC, Brg1(II), npBAF and nBAF.
Positive Control
Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for SMARCC1 / BAF155 antibody.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
