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PBRM1 antibody (pAb)
| RRID: AB_2793612 |
| Aliases: PB1, BAF180 |
| Catalog No: 61381 | Format: 100 µl | ¥5,830 | Add to Cart |
| Catalog No: 61382 | Format: 10 µl | ¥1,430 | Add to Cart |
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Applications
Application Notes
Applications Validated by Active Motif:
ChIP-Seq: 7 µl (4 µg) per ChIP
WB: 1:500 - 1:1,000 dilution
Addition of 0.05% Tween 20 in blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
ChIP-Seq, ChIP-reChIP
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This antibody was raised against a peptide within the N-terminal region of human PBRM1.
Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.
PBRM1 antibody (pAb) tested by ChIP-Seq.Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from mouse liver and 4 µg PBRM1 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 3.9 million sequence tags were mapped to identify PBRM1 binding sites. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.
PBRM1 antibody (pAb) tested by Western blot.
The analysis was performed using 40 µg of A-431 nuclear extract (Cat. No. 36004) and PBRM1 (pAb) at a 1:1000 dilution.
Background
PBRM1 (polybromo 1) is a component of the SWI/SNF-B (PBAF) ATP-dependent chromatin-remodeling complex. It is involved in transcriptional activation and repression of select genes by chromatin remodeling and is an integral component of complexes necessary for ligand-dependent transcriptional activation by nuclear hormone receptors. Defects in PBRM1 have been associated with primary clear cell renal cell carcinoma.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with PBRM1 antibody (pAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
