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RNA Pol II CTD phospho Thr4 antibody (mAb)
| RRID: AB_2750848 |
| Clone: 6D7 |
| Aliases: RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220 |
| Catalog No: 61361 | Format: 100 µg | ¥5,830 | Add to Cart |
| Catalog No: 61362 | Format: 10 µg | ¥1,430 | Add to Cart |
| Catalog No: 61961 | Format: 50 µg | ¥3,360 | Add to Cart |
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Applications
Application Notes
Applications Validated by Active Motif:
WB: 0.5 - 2 µg/ml
DB: 1 µg/ml
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
ChIP-qPCR
IP
ICC/IF
WB
ELISA
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This antibody was raised against a synthetic peptide corresponding to RNA Pol II CTD phosphorylated at threonine 4.
Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.
Western blot of RNA pol II CTD phospho Thr4 mAb.
30 µg of HeLa cell nuclear extract was probed with RNA pol II CTD phospho Thr4 antibody at 2 µg/ml in buffer containing 0.05% Tween.
RNA pol II CTD phospho Thr4 antibody tested by bead-based assay
Luminex bead-based specificity analysis was used to confirm the specificity of RNA pol II CTD phospho Thr4 antibody for RNA pol II CTD peptides conjugated to MagPlex Luminex beads and incubated with 50ng of RNA pol II CTD phospho Thr4 antibody. Peptide-bound antibody was detected with anti-mouse IgG-Phycoerythrin and read in a Luminex instrument. (Luminex® is a registered trademark of Luminex Corporation.)
Dot blot analysis was used to confirm the specificity of RNA Pol II phospho Thr4 antibody
10, 50, and 250 pmoles of RNA Pol II CTD were spotted onto nitrocellulose and probed with the antibody at 1 µg/ml. Lane 1: RNA Pol II CTD phospho-Thr4. Lane 2: RNA Pol II CTD Thr4 (unmodified)
Background
RNA pol II (RNA polymerase II) is a complex of 12 proteins that participates in transcription, mRNA processing, and transcription-coupled DNA repair. RNA polymerase II is distinguished by its large carboxyl-terminal repeat domain (CTD), composed of repeats of the consensus heptapeptide Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Polo-like kinase 3 (plk3) has been reported to phosphorylate Thr4 which may be required for transcriptional elongation.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with RNA Pol II CTD phospho Thr4 antibody (mAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
