SMARCAL1 / HARP antibody (mAb)

RRID: AB_2614999
Clone: 4B10
Aliases: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1, HepA-related protein, DNA-dependent ATPase A
Catalog No: 61115 Format: 100 µg ¥3,630 Add to Cart
Catalog No: 61116 Format: 10 µg ¥1,210 Add to Cart

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
150 kDa
Reactivity:
Bovine, Human, Mouse

Applications

Western Blot Validated Immunoprecipitation Validated

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

IP
WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This SMARCAL1 / HARP antibody was raised against purifed bovine SMARCAL1 / HARP protein.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

Background

SMARCAL1 / HARP (SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1, HepA-related protein, DNA-dependent ATPase A) is a member of the SWI / SNF family of ATPases and chromatin remodeling proteins. Unlike other SWI / SNF family members, SMARCAL1 / HARP has not been shown to remodel chromatin, but possesses an ATP-dependent annealing helicase activity. SMARCAL1 reanneals single-stranded DNA bubbles in DNA at DNA replication forks that are bound by Replication Protein A (RPA). Defects in SMARCAL1 / HARP are a cause of Schimke immuno-osseous dysplasia (SIOD) SIOD causes spondyloepiphyseal dysplasia (a disorder of bone growth that results in short stature, skeletal abnormalities, and problems with vision and hearing), renal dysfunction and T-cell immunodeficiency.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting