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MeCP2 phospho Ser80 antibody (pAb)
| RRID: AB_2793324 |
| Aliases: RS, RTS, RTT, PPMX, MRX16, MRX79, MRXSL, AUTSX3, MRXS13 |
| Catalog No: 39733 | Format: 100 µl | ¥5,670 | Add to Cart |
| Catalog No: 39734 | Format: 10 µl | ¥1,430 | Add to Cart |
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Applications
Application Notes
Applications Validated by Active Motif:
WB: 1:2,000 - 1:10,000 dilution
Immunogen
This MeCP2 phospho Ser80 antibody was raised against a peptide containing phospho Ser80 of mouse MeCP2.
Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
MeCP2 phospho Ser80 antibody (pAb) tested by Western blot.
Detection of MeCP2 phospho Ser80 by Western blot analysis. Rat brain extract (20 µg) probed with MeCP2 phospho Ser80 antibody (pAb) at a 1:5,000 dilution.
Background
Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as development and control of gene expression. It is generally associated with the repressive chromatin state. The complex series of events leading to this repressive state involve the coordinated regulation of DNA methyltransferases and two other groups of proteins called the Methyl-CpG binding proteins (MBD proteins) and the Kaiso family of proteins. The MBD family of proteins include MeCP2, MBD1, MBD2, MBD3 and MBD4. MeCP2 (methyl-CpG binding protein 2)can repress transcription by recruiting the Sin3 complex including histone deacetylases (HDACs). MeCP2 is distributed throughout the nucleus in human cells. Phosphorylation of MeCP2 at serine 80 may regulate the activity of neurons switching between resting and active states in neural circuits.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
