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Ring1B antibody (mAb)
| RRID: AB_2615006 |
| Clone: 3-3 |
| Aliases: RNF2, BAP1, DING, BAP-1, HIPI3, RING2 |
| Catalog No: 39663 | Format: 100 µg | ¥5,830 | Add to Cart |
| Catalog No: 39664 | Format: 10 µg | ¥1,430 | Add to Cart |
| Catalog No: 39063 | Format: 50 µg | ¥3,360 | Add to Cart |
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Applications
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
ChIP, ChIP-Seq
IP
ICC/IF
WB
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This Ring1B antibody was raised against the recombinant full-length mouse protein.
Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Ring1B antibody (mAb) tested by ChIP-chip.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from 4.5 million human embryonic stem cells and 4 µl of antibody. ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The two images show regions of strong binding on chromosomes 2 and 9.
Ring1B antibody (mAb) tested by ChIP-Seq.
ChIP was performed using Ring1B antibody with 30 µg chromatin from the wild-type mouse ESC and 4 µg of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 19.5 million sequence tags were mapped to identify Ring1B binding sites. The image shows binding across a region of chromosome 2.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Ring1B antibody (mAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
