Histone macroH2A1 antibody (pAb)

Application Notes

Applications Validated by Active Motif:
ICC/IF: 1:250 - 1:1,000 dilution
WB: 1:1,000 - 1:2,500 dilution

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

WB
IF

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This Histone macroH2A1 antibody was raised against recombinant protein corresponding to the N-terminal half of mouse macroH2A1.2, so will react with macroH2A1 and the macroH2A1.1 and macroH2A1.2 isoforms.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

Background

Histone macroH2A (mH2A) is a histone variant that has a region that is similar to histone H2A but has a unique C-terminal domain (the macro domain, also called the non-histone domain (NHD)) in addition to the histone-like region. mH2A associates with condensed chromatin, including the inactive mammalian female X chromosome, senescence-associated heterochromatin foci, imprinted genetic loci, and regions of chromatin that are CpG methylated. In vitro assays with mH2A have shown that nucleosomes containing macroH2A are resistant to ATP-dependent chromatin remodeling and transcription factor binding, and that macroH2A is capable of repressing the initiation of RNA pol II catalyzed transcription.

MacroH2A1 consists of two isoforms, macroH2A1.1 and macroH2A1.2, that are produced by alternative splicing; they differ by only 30 amino acids in the C-terminal non-histone domain (NHD).

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone macroH2A1 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

• ChIP = Chromatin Immunoprecipitation;
• ChIP = ChIP Sequencing;
• CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
• CUT&Tag = Cleavage Under Targets and Tagmentation;
• DB = Dot Blot;
• ELISA = Enzyme-linked Immunosorbent Assay;
• EMSA = Electrophoretic Mobility Shift Assay
• FC = Flow Cytometry;
• ICC = Immunocytochemistry;
• IF = Immunofluorescence;
• IHC = Immunohistochemistry;
• IP = Immunoprecipitation;
• MeDIP = Methyl-DNA Immunoprecipitation;
• NEU = Neutralizing;
• WB = Western Blotting