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HP1α antibody (pAb)
| RRID: AB_2255953 |
| Aliases: CBX5, HP1, Hp1a |
| Catalog No: 39295 | Format: 200 µl | ¥5,670 | Add to Cart |
| Catalog No: 39296 | Format: 10 µl | ¥1,430 | Add to Cart |
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Applications
Application Notes
Applications Validated by Active Motif:
WB: 1:500 - 1:1,000 dilution
Immunogen
This HP1 alpha antibody was raised against a recombinant protein corresponding to full-length recombinant mouse HP1 alpha.
Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
HP1 alpha pAb tested by Western blot.
Detection of HP1 alpha by Western blot analysis. Nuclear extract of HeLa cells (20 µg/lane) stained with HP1 alpha pAb at a 1:1,000 dilution.
Background
HP1 (Heterochromatin protein 1) is a chromosomal protein that participates in both chromatin packaging and gene silencing. Three HP1 isoforms (α, β and γ) occur in mammals. The chromodomain (Chromatin Organization Modifier) of HP1α is responsible for binding to Histone H3 methyl Lys9, although additional factors are required to specify the HP1 targeting. The chromoshadow domain mediates interactions with a number of nuclear proteins, including SuvH39 (a Histone H3 Lysine methyltransferase), the DNA methyltransferases DNMT1 and DNMT3A or the large subunit of the chromatin assembly factor-1 (CAF-1). Dysfunction of HP1α plays a critical role in tumorigenesis, and is implicated in the invasive/metastatic phenotype of breast cancer.
Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for HP1 alpha antibody.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
