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Recombinant SMYD2 protein
| Aliases: KMT3C, HSKM-B, ZMYND14 |
| Catalog No: 31497 | Format: 20 µg | ¥3,760 | Add to Cart |
| Catalog No: 31901 | Format: 1 mg | ¥33,200 | Add to Cart |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
SMYD2 (SET and MYND domain containing protein 2) is a lysine methyltransferase that methylates both histones and non-histone proteins. Specifically, SMYD2 methylates histone H3 Lys4 (H3K4me1) and dimethylates histone H3 Lys36 (H3K36me2). SMYD2 also exhibits methyltransferase activity toward non-histone proteins such as p53 and pRb. SMYD2 monomethylates Lys370 of p53, leading to decreased DNA-binding activity and subsequent inhibition of p53-mediated transcriptional regulation. SMYD2 also monomethylates Lys860 of pRb.
Application Notes
Recombinant SMYD2 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
HMT Assay Conditions: 2 ug Calf Thymus Octamer was incubated with different concentrations of recombinant SMYD2 protein in reaction buffer containing 50 mM TrisCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 100 μM 3H-SAM for 3 hours at room temperature. Activity was detected by Autoradiography.
Protein Details
Recombinant SMYD2 protein (accession number NP_064582.2) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with a molecular weight of 50.7 kDa. The recombinant protein is >90% pure by SDS-PAGE.
Recombinant SMYD2 protein gel
SMYD2 protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.
Recombinant SMYD2 protein activity assay
2 ug Calf Thymus Octamers was incubated with 0.5 ug and 1 ug SMYD2 protein respectivley in reaction buffer containing radioactive 3H-SAM for 3 hours at room temperature. Activity was detected by Autoradiography.
*Our result showed that H4 may also be methylated by SMYD2.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
