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Recombinant KAT7 protein
| Aliases: Lysine Acetyltransferase 7, HBO1, HBOA, MYST2, Histone Acetyltransferase Binding To ORC1 |
| Catalog No: 31489 | Format: 20 µg | ¥4,040 | Add to Cart |
| Catalog No: 31889 | Format: 1 mg | ¥33,200 | Add to Cart |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
KAT7 (K(Lysine) Acetyltransferase 7) is a MYST-family histone acetyltransferase (HAT) that forms a complex with ING4/5, JADE1/2/3, and Eaf6, which is found enriched on coding regions of genes, supporting a role in transcriptional elongation. In addition, KAT7 is thought to participate in events controlling the initiation of DNA replication.
Application Notes
Recombinant KAT7 protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.
Protein Details
Full length recombinant KAT7 protein (accession number NP_001186084.1) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with a molecular weight of 67.2 kDa. The recombinant protein is >85% pure by SDS-PAGE.
Recombinant KAT7 protein
10% SDS-PAGE Coomassie staining
MW: 67.2 kDa
Purity: ≥ 95%
Recombinant KAT7 protein activity assay
0.5 µg of Histone H4 (Cat# 31493) was incubated with 0 μg, 0.05 µg, 0.1 µg, and 0.2 µg KAT7 protein in 20 µl reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 20 uM acetyl-CoA for 2 hr at room temperature. 2.5 µl of reaction products were run on a 12.5% SDS-PAGE and detected by H4ac-pan antibody (Cat# 39243).
Western Blot was used for activity detection.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
