Recombinant SMYD1 protein

Aliases: BOP; KMT3D; ZMYND18; ZMYND22
Catalog No: 31405 Format: 20 µg ¥4,160 Add to Cart
Catalog No: 31805 Format: 1 mg ¥36,000 Add to Cart

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Expressed In: Baculovirus Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

SET and MYND domain-containing protein 1 (SMYD1) is a member of the SMYD family of histone methyltransferases that possesses H3K4 methyltransferase activity. It has also been shown to act as a transcriptional repressor. Studies suggest that SMYD1 may play a critical role in cardiomyocyte differentiation and cardiac morphogenesis and that it also may have a function in skeletal muscle development.

Application Notes

Recombinant SMYD1 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Specific Activity: Not known. May methylate non-histone substrates.

Protein Details

Recombinant SMYD1 (accession number NP_938015.1) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with an observed molecular weight of 58.3 kDa. The recombinant protein is >85% pure by SDS-PAGE.

References


This product was used in the following publications:
Mol. Cell. (2016). 61(2): 247-59. PMID: 26778125. (Histone Methyltransferase Assay)

 
SMYD1 Coomassie gel

Recombinant SMYD1 protein gel.
SMYD1 protein was run on an SDS-PAGE gel and stained with Coomassie blue.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.