Recombinant NSD1-SET protein

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

NSD1 (Nuclear Receptor Binding SET Domain Protein 1) is a histone methyltransferase that preferentially methylates lysine 36 of histone H3 and lysine 20 of histone H4 in vitro. NSD1 is a transcriptional intermediary factor capable of both negatively or positively influencing transcription, depending on the cellular context. NSD1 protein contains a SET domain, 2 LXXLL motifs, 3 nuclear translocation signals (NLSs), 4 plant homeodomain (PHD) finger regions, and a proline-rich region. The encoded protein enhances androgen receptor (AR) transactivation, and this enhancement can be increased further in the presence of other androgen receptor-associated coregulators. This protein may act as a nucleus-localized, basic transcriptional factor as well as a bifunctional transcriptional regulator. Mutations of this gene have been associated with Sotos syndrome and Weaver syndrome. One version of childhood acute myeloid leukemia is the result of a cryptic translocation with the breakpoints occurring within nuclear receptor-binding Su(var), Enhancer of zeste (EZH2), and Trithorax Domain Protein 1 on chromosome 5 and Nucleoporin, 98 kd on chromosome 11.
NSD1-SET includes the SET domain of NSD1. The SET domain is a 130 to 140 amino acid, evolutionary conserved motif that was initially characterized in the Drosophila proteins Su(var)3-9, EZH2 and TrxG and plays a crucial role in substrate recognition and enzymatic activity.

Application Notes

Recombinant NSD1-SET protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

HMT Assay Conditions: 30 ng/μl recombinant nucleosomes were incubated with 30 ng/μl recombinant NSD1-SET protein in reaction buffer including 50 mM TrisCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 9 nCi/μl 3H-SAM for 2 hours at room temperature. Activity was detected by fluorography.

Protein Details

Recombinant NSD1-SET peptide that includes amino acids 1852-2082 that contains the SET domain sequence of the human NSD1 protein (accession number NP_071900.2) was expressed in E. coli cells and contains an N-terminal GST-Tag with an observed molecular weight of 51 kDa. The recombinant protein is >95% pure by SDS-PAGE.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.