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Expressed In: E. coli Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
PTPN2 (Protein Tyrosine Phosphatase, Non-Receptor Type 2, also known as TCELLPTP, TCPTP, TC- PTP, PTPT, PTN2) is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family have a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation as signaling molecules. Epidermal growth factor receptor (EGFR) and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. PTPN2 dephosphorylates multiple substrates including receptor protein tyrosine kinases INSR, EGFR, CSF1R, PDGFR and non-receptor protein tyrosine kinases like JAK1, JAK2, JAK3, Src family kinases, STAT1, STAT3, STAT5A, STAT5B and STAT6 either in the nucleus or the cytoplasm.
Application Notes
This protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Activity Assay Conditions: 1 mM pNPP was incubated with different concentrations of PTPN2 protein in reaction buffer including 50 mM HEPES pH 7.5, 2 mM EDTA, 3 mM DTT, 100 mM NaCl at room temperature. The absorbance at 405 nm was continuously monitored for 30 minutes.
Protein Details
Recombinant PTPN2 was expressed in E. coli cells as the full length protein (accession number NP_002819.2) with an N-terminal GST-Tag. The molecular weight of the protein is 75.7 kDa.
Recombinant PTPN2 protein gel.
Recombinant PTPN2 protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.
MW: 75.7 kDa
Purity: > 50%
Recombinant PTPN2 protein activity assay.
1 mM pNPP was incubated with different concentrations of PTPN2 protein in reaction buffer including 50 mM HEPES pH 7.5, 2 mM EDTA, 3 mM DTT, 100 mM NaCl at room temperature. The absorbance at 405 nm was continuously monitored for 30 minutes.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
