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Recombinant SETD8 protein
| Aliases: SET8, KMT5A, SET07, PR-Set7 |
| Catalog No: 31427 | Format: 20 µg | ¥4,040 | Add to Cart |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
SETD8 (SET Domain Containing (Lysine Methyltransferase) 8) is Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins.It specifically catalyzes monomethylation of histone H4 lysine 20 (H4K20) which is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. H4K20me0 is also involved in DNA damage response. Besides H4K20, SETD8 monomethylates non-histone substrates including proliferating cell nuclear antigen (PCNA) and promotes carcinogenesis by deregulating PCNA expression. It also mediates monomethylation of p53/TP53 at 'Lys-382'and leads to repress p53/TP53-target genes.
Application Notes
Recombinant SETD8 protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.
Protein Details
Full length recombinant SETD8 protein (accession number NP_065115.3) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with a molecular weight of 42.2 kDa. The recombinant protein is >85% pure by SDS-PAGE.
Recombinant SETD8 protein gel.
Recombinant SETD8 was run on an 8% SDS-PAGE gel and stained with Coomassie blue.
Recombinant SETD8 protein activity assay.
Recombinant SETD8 activity titration was assessed using a Radiometry assay. Titration curves were generated to show signal response in the presence of histone peptide substrate at increasing SETD8 concentrations. This data was generated and kindly provided courtesy of ChemPartner.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
