Recombinant NFκB1 p105 protein

Catalog No: 81143 Format: 20 µg ¥3,800 Buy
Catalog No: 81843 Format: 1 mg ¥32,400 Buy

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Expressed In: Baculovirus Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

NFκB1 (Nuclear Factor Kappa B Subunit 1), also known as NFKB1 or NF-kappa-B, is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. The 105 kD protein can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. It is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB1 is a homo- or heterodimeric complex formed by the Rel-like domaincontaining proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFκB1/p105 also appears to occur post-translationally.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Recombinant NF:kappa;B1 / NFκB p105 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_003989.2) with an N-terminal FLAG tag. The molecular weight of the protein is 107.1 kDa.

 
Recombinant NFKB1 / NFκB p105 protein gel

Recombinant NFKB1 / NFκB p105 protein gel
7.5% SDS-PAGE Coomassie staining
MW: 107.1 kDa
Purity: >85%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.