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Recombinant SUV420H1 (2-387) protein
| Catalog No: 81021 | Format: 50 µg | ¥3,800 | Buy |
| Catalog No: 81721 | Format: 1 mg | ¥24,000 | Buy |
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Expressed In: E. coli Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
SUV420H1 (Suppressor Of Variegation 4-20 Homolog 1, also known as KMT5B) is a histone methyltransferase that specifically trimethylates nucleosomal histone H4 on lysine 20 (H4K20). H4K20me3 is a histone marker that represents epigenetic transcriptional repression. SUV420H1 mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H1 is targeted to histone H3 via its interaction with RB1 family proteins. It plays a role in myogenesis by regulating the expression of target genes, such as EID3.
Application Notes
Recombinant SUV420H1 (2-387) is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
HMT Assay Conditions: 2 µg Recombinant Nucleosomes were incubated with different concentrations of SUV420H1 (2- 387) protein in 30 µl reaction system containing 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, and 50 µM SAM for 3 hours at room temperature. Western Blot was used to detect the generation of reaction products.
Protein Details
Recombinant SUV420H1 (2-387) protein corresponding to amino acids 2-387 of SUV420H1 protein (accession number NP_060105.3) was expressed in E. coli cells and contains an N-terminal GST tag with a molecular weight of 71.1 kDa. Recombinant SUV420H1 (2-387) protein contains the SET domain of SUV420H1 that is responsible for methyltransferase activity.
Recombinant SUV420H1 (2-387) protein gel.
10% SDS-PAGE gel stained with Coomassie blue.
MW: 71.1 kDa
Purity: > 75%
Recombinant SUV420H1 (2-387) activity assay
2 µg Recombinant Nucleosomes (H3.1) (Cat. No. 31466) were incubated with 0 µg (-), 0.2 µg (+), 0.4 µg (++), 0.8 µg (+++) SUV420H1 (2-387) protein, respectively, in 30 µl reaction system containing 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 µM SAM for 3 hr at room temperature. 6 µl reaction products were loaded and run on a 12.5% SDS-PAGE gel. Western Blot was used to detect the generation of reaction products (H4K20me3 antibody, Cat. No. 39672, 1:1000 dilution). SUV420H1 (2-387) only was used as negative control.
The Western blot result shows that nucleosomes are much better than histone octamers as the substrate of SUV420H1 (2-387).
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
