CTBP2 antibody (pAb)

RRID: AB_2793573
Catalog No: 61261 Format: 100 µl ¥5,610 Buy
Catalog No: 61262 Format: 10 µl ¥1,380 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
50 kDa
Reactivity:
Human, Mouse

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated

Application Notes

Applications Validated by Active Motif:
ChIP-Seq: 4 µg each
ChIP: 10 µl per ChIP
WB*: 1:500 - 1:1,000 dilution

*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This CTBP2 antibody was raised against a peptide in the C-terminal region of human CTBP2. This antibody recognizes both isoform 1 and 2.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 
ChIP-Seq analysis of CTBP2 (pAb)

CTBP2 (pAb) tested by ChIP-Seq
ChIP was performed using 30 µg of chromatin from the HepG2 cell line and 4 µg of antibody. ChIP DNA was sequenced on the Illumina NextSeq 500 and 12 million sequence tags were mapped to identify CTBP2 binding sites. The image shows binding across a region of chromosome 2. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

CTBP2 antibody (pAb) tested by Western blot.

CTBP2 antibody (pAb) tested by Western blot
Nuclear extract of HeLa cells (30 µg) probed with CTBP2 antibody (1:500).

Background

The CTBP2 (C-terminal binding protein 2) gene encodes a protein that is a corepressor targeting diverse transcription regulators. It functions in brown adipose tissue (BAT) differentiation. Isoform 2 probably acts as a scaffold for specialized synapses. Isoform 2 is also phosphorylated upon DNA damage, probably by ATM or ATR at Thr-179; Ser-181 and Ser-185. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for CTBP2 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with CTBP2 antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting