EKLF antibody (mAb)

RRID: AB_2615069
Clone: 7B2
Aliases: KLF1, INLU, CDAN4, HBFQTL6
Catalog No: 61233 Format: 100 µg ¥3,520 Buy
Catalog No: 61234 Format: 10 µg ¥1,160 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG3
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
42 kDa
Reactivity:
Mouse

Applications

ChIP Validated

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP-qPCR
Slot blot

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

This product is currently on indefinite backorder.

Immunogen

This EKLF antibody was raised against a full-length recombinant protein to mouse EKLF (Kruppel-like factor 1 (erythroid)).

Buffer

Purified IgG in 12 mM Tris, pH 8.0, 60 mM Na Citrate, 120 mM NaCl with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

Background

The EKLF (Erythroid krueppel like transcription factor, Erythroid transcription factor, KLF1) gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with EKLF antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting