CENP-E antibody (mAb)
RRID: AB_2793278 |
Clone: 1H12 |
Aliases: KIF10, CENPE, PPP1R61 |
Catalog No: 39619 | Format: 200 µg | ¥3,520 | Buy |
Catalog No: 39620 | Format: 10 µg | ¥1,160 | Buy |
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Applications
Application Notes
Applications Validated by Active Motif:
ICC/IF: 1 µg/ml dilution
This CENP-E antibody can be used in Western blot to detect CENP-E if the protein has been first enriched by immunoprecipitation.
Immunogen
This CENP-E antibody was raised against full-length human CENP-E protein.
Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Background
Centrosome-associated protein E (CENP-E) is a kinesin-like protein and an integral component of kinetochore fibers that link centromeres to spindle microtubules. CENP-E localizes to kinetochores throughout all phases of mitotic chromosome movement. After the onset of anaphase it is released, and by telophase becomes bound to interzonal microtubules of the mitotic spindle. It is likely that CENP-E serves as a chromosome motor during anaphase.
Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for CENP-E antibody (Clone 1H12).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting