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2 x 100 µg of COS-7 nuclear extract (IFNγ treated) at 2.5 µg/µl.
Background
COS-7 nuclear extract (IFNγ treated) was prepared from subconfluent undifferentiated cell cultures of the Simian kidney fibroblast COS-7 cell line. The COS-7 cell line was derived from immortalizing the African green monkey CV-1 cell line by transformation with an origin-defective mutant of the SV40 viral genome that is replication-deficient but produces wild-type large T antigen. The large T antigen supports replication of and lytic growth of the SV40 virus as well as replication of SV40 promoter-containing plasmids. In addition to supporting propagation of the Simian SV40 virus, the COS-7 cell line is most commonly used in research related to replication and in transfection studies for the production of recombinant proteins.
Treatment of COS-7 cells with IFNγ promotes apoptosis, causes alterations in viral replication efficiency, and induces the JAK/STAT signaling pathway.
Application Notes
COS-7 nuclear extract (IFNγ treated) is specifically recommended for 1) analysis of replication, 2) for transfection studies, and 3) studies of apoptosis.
Extract Origin
Monkey Kidney, SV40 transformed
Extract Composition
COS-7 nuclear extract is supplied in Dilution Buffer (20 mM Hepes pH 7.5, 400 mM NaCl, 20% glycerol, 0.1 mM EDTA, 10 mM NaF, 10 µM Na2MoO4, 1 mM NaVO3, 10 mM PNPP, 10 mM β-glycerophosphate, 1 mM DTT and protease inhibitors). Cells were treated with 1000 U/ml of IFNγ for 15 minutes immediately prior to harvesting.
Quality Control
Each lot has been tested for STAT activation by using TransAM® Kits.
Storage
To ensure stability, extracts should be stored at -80°C.
We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
