Recombinant WTAP protein

Catalog No: 31571 Format: 20 µg ¥3,880 Buy

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Expressed In: Baculovirus Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

N6-methylated adenine (m6A) is prevalently present in nearly all RNA types and can be found in all organisms from bacteria to humans. It preferentially appears around stop codons and within long internal exons in mammalian messager RNAs. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. m6A also takes place in other RNA molecules, such as primary miRNA (pri-miRNAs).
WTAP (Wilms Tumor 1 Associated Protein, as known as Mum2) is a ubiquitously expressed nuclear protein. WTAP is the regulatory subunit of the WMM N6-methyltransferase complex, a multiprotein complex that mediates N6-methyladenosine (m6A) methylation of some adenosine residues of some mRNAs. WTAP is required for accumulation of METTL3 and METTL14 to nuclear speckle. It acts as a mRNA splicing regulator. WTAP also regulates G2/M cell-cycle transition by binding to the 3’ UTR of CCNA2, which enhances its stability. WTAP can impair WT1 DNA-binding ability and inhibit expression of WT1 target genes.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Recombinant WTAP protein was expressed in a baculovirus expression system as the full length protein (accession number NP_004897.2) with an N-terminal FLAG-Tag. The molecular weights of WTAP is 45.2 kDa.

 

Recombinant WTAP protein gel.
Recombinant WTAP protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.