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Recombinant MAOA protein
| Aliases: Monoamine Oxidase A, MAO-A |
| Catalog No: 31502 | Format: 10 µg | ¥3,200 | Buy |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
MAOA (Monoamine Oxidase A) is one of two neighboring mitochondrial enzyme family members which catalyze the oxidative deamination of amines, such as dopamine, norepinephrine, and serotonin. The other member is MAOB. Mutation of MAOA results in Brunner syndrome and maoa-related behavior disorders. MAOA has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine.
Application Notes
Recombinant MAOA is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Monoamine Oxidase Assay: 40 µM substrate was incubated with MAOA protein in reaction buffer for 1 hour at room temperature, then the product was incubated with Detection Reagent for 0.5 hour at temperature. The activity of MAOA was detected by Promega MAO-Glo Assay.
Protein Details
Recombinant MAOA (accession number NP_000231.1) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with an observed molecular weight of 60.7 kDa. The recombinant protein is >80% pure by SDS-PAGE.
References
The following applications have been published using this product. Active Motif may not have validated the protein for use in these applications:
LSD1 Inhibition
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use those products.
Recombinant MAOA protein gel.
MAOA protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.
MAO-GloTM Assay for MAOA activity
40 µM substrate was incubated with MAOA protein in reaction buffer for 1 hour at room temperature, then the product was incubated with Detection Reagent for 0.5 hour at temperature. The activity of MAOA was detected by Promega MAO-Glo Assay.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
