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AbFlex® Recombinant Antibodies

defined antibodies for highly specific, reproducible performance

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. The unique advantages of the AbFlex® antibody are its flexible labeling and purification options. Each AbFlex® antibody contains a Sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner using our Sortag-IT Labeling Kits. Every antibody also contains a 6xHis tag, which can be used with nickel-based purification systems, and an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.

AbFlex antibody with flexible labeling and purification options
AbFlex® recombinant antibody (rAb) with flexible labeling and purification options.

 

AbFlex® antibodies are specifically labeled at the end of the constant region of the heavy chain to avoid interference with antigen recognition and functionality. This is important as it ensures the labeling process maintains the integrity of the antibody so signal is not diminished as a result of non-functional antibodies. In contrast, commonly used chemical labeling methods add labels to the antibody in a random fashion. The randomness of this process has a high potential to block the antigen-binding site and render the antibody ineffective. Chemical labeling can also deposit labels on the Fc region of the antibody which has the potential to obstruct interactions with protein A.

AbFlex® directed labeling versus chemical labeling
Differences in label distribution between AbFlex® direct labeling and chemical labeling.

 

Prior to the development of recombinant antibodies, researchers relied on polyclonal and monoclonal antibodies. Polyclonal antibodies recognize multiple epitopes on the same antigen. This increases antibody affinity, but often has a higher potential for cross-reactivity. Monoclonal antibodies represent a single antibody producing B cell that binds to one unique epitope. However, monoclonal antibodies can be lost or evolve over time.

Unlike monoclonal antibodies, which can experience gene loss, mutations and cell-line drift that alter antibody performance over time and lead to variability across antibody lots, recombinant antibodies are highly consistent because they contain the same immunoglobulin heavy and light chain DNA sequences in every lot. This provides batch-to-batch reproducibility in antibody performance and eliminates the need to re-evaluate or compare different antibody lots, thereby, saving valuable time and effort on assay development.

To ensure optimal performance, each AbFlex® antibody is carefully screened for specificity and sensitivity. Only heavy and light chain sequences that produce highly specific antibodies, as verified in our bead-based binding assay, are selected. Antibodies that utilize the AbFlex® direct labeling approach also show greater sensitivity than their parent monoclonal antibodies across a variety of applications.

Another advantage of AbFlex® recombinant antibodies is the fact that they are produced outside of an animal system. Polyclonal antibodies rely on an animal’s immune response and epitope specificity, both of which are highly variable across animals and immunizations. Polyclonal antibodies are also hindered by having a finite supply, making it difficult to perform long-term antibody projects or reproduce data over time or across labs. With AbFlex® recombinant antibodies, the full-length IgG is expressed in a mammalian system to ensure a consistent, stable antibody supply. Additionally, any ethical or animal welfare concerns associated with antibody production and maintenance are avoided.

Advantages of AbFlex® Recombinant Antibodies (rAbs)

  • Flexible labeling & purification options
  • Consistent performance across lots
  • Highly specific and sensitive antibodies
  • Reliable, long-term supply
  • Animal-free production

The following list contains Active Motif's AbFlex® recombinant antibodies (rAbs). For information about site-specific labeling of the AbFlex® antibodies, please visit our collection of Sortag-IT™ Labeling Kits.

 
Name Isotype Reactivity Appl. Format Cat No. Price  
AbFlex™ 5-methylcytosine antibody (rAb) IgG2a Other (Not Species Specific) DB 100 µg 91187 ¥5,438 Buy Now
10 µg 91188 ¥1,088 Buy Now
AbFlex™ BMI-1 antibody (rAb) IgG2a Human WB 100 µg 91195 ¥5,438 Buy Now
10 µg 91196 ¥1,088 Buy Now
AbFlex™ Cas9 antibody (rAb) IgG2a Human ELISA, WB 100 µg 91123 ¥5,438 Buy Now
10 µg 91124 ¥1,088 Buy Now
AbFlex™ EED antibody (rAb) IgG2a Human ChIP, ChIP-Seq, ELISA 100 µg 91135 ¥5,438 Buy Now
10 µg 91136 ¥1,088 Buy Now
AbFlex™ HDAC2 antibody (rAb) IgG2a Human WB 100 µg 91197 ¥5,438 Buy Now
10 µg 91198 ¥1,088 Buy Now
AbFlex™ HIF-1 alpha antibody (rAb) IgG2a Bovine, Human, Mouse, Rat ELISA 100 µg 91139 ¥5,438 Buy Now
10 µg 91140 ¥1,088 Buy Now
AbFlex™ His Tag antibody (rAb) IgG2a Other (Not Species Specific) WB 100 µg 91183 ¥5,438 Buy Now
10 µg 91184 ¥1,088 Buy Now
AbFlex™ AM-Tag antibody (rAb) IgG2a Other (Wide Range) ChIP, ELISA, WB 100 µg 91111 ¥5,438 Buy Now
10 µg 91112 ¥1,088 Buy Now
AbFlex™ AM-Tag antibody (rAb) (biotin) IgG2a Other (Wide Range) ChIP, ELISA, WB 100 µg 91109 ¥5,873 Buy Now
10 µg 91110 ¥1,233 Buy Now
AbFlex™ Histone H3K9me0 Antibody (rAb) IgG2a Human, Other (Wide Range) ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91155 ¥5,438 Buy Now
10 µg 91156 ¥1,088 Buy Now
AbFlex™ Histone H3K9ac antibody (rAb) IgG2a Human, Other (Wide Range) ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91103 ¥5,438 Buy Now
10 µg 91104 ¥1,088 Buy Now
AbFlex™ Histone H3K9ac antibody (rAb) (biotin) IgG2a Human, Other (Wide Range) ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91101 ¥5,873 Buy Now
10 µg 91102 ¥1,233 Buy Now
AbFlex™ Histone H3K27ac antibody (rAb) IgG2a Human ELISA, WB 100 µg 91193 ¥5,438 Buy Now
10 µg 91194 ¥1,088 Buy Now
AbFlex™ Histone H3K56ac antibody (rAb) IgG2a Human, Other (Wide Range) ChIP-Seq, ELISA 100 µg 91127 ¥5,438 Buy Now
10 µg 91128 ¥1,088 Buy Now
AbFlex™ Histone H3.3 antibody (rAb) IgG2a Human WB 100 µg 91191 ¥5,438 Buy Now
10 µg 91192 ¥1,088 Buy Now
AbFlex™ Histone H4K20me3 antibody (rAb) IgG2a Human, Other (Wide Range) ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91107 ¥5,438 Buy Now
10 µg 91108 ¥1,088 Buy Now
AbFlex™ Histone H4K20me3 antibody (rAb) (biotin) IgG2a Human, Other (Wide Range) ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91105 ¥5,873 Buy Now
10 µg 91106 ¥1,233 Buy Now
AbFlex™ JMJD2A antibody (rAb) IgG2a Human ELISA, WB 100 µg 91143 ¥5,438 Buy Now
10 µg 91144 ¥1,088 Buy Now
AbFlex™ Lamin A/C antibody (rAb) IgG2a Human WB 100 µg 91189 ¥5,438 Buy Now
10 µg 91190 ¥1,087.50 Buy Now
AbFlex™ MAZ antibody (rAb) IgG2a Human WB 100 µg 91205 ¥5,438 Buy Now
10 µg 91206 ¥1,088 Buy Now
AbFlex™ Myc-Tag antibody (rAb) IgG2a Human WB 100 µg 91203 ¥5,438 Buy Now
10 µg 91204 ¥1,088 Buy Now
AbFlex™ c-Myc antibody (rAb) IgG2a Human WB 100 µg 91213 ¥5,438 Buy Now
10 µg 91214 ¥1,088 Buy Now
AbFlex™ RNA Pol II antibody (rAb) IgG2a Human ChIP-Seq, IF, WB 100 µg 91151 ¥5,438 Buy Now
10 µg 91152 ¥1,088 Buy Now
AbFlex™ RNA pol II CTD phospho Ser2 antibody (rAb) IgG2a Human ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91115 ¥5,438 Buy Now
10 µg 91116 ¥1,088 Buy Now
AbFlex™ RNA pol II CTD phospho Ser5 antibody (rAb) IgG2a Human ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91119 ¥5,438 Buy Now
10 µg 91120 ¥1,088 Buy Now
AbFlex™ RNA pol II CTD phospho Ser5 antibody (rAb) (biotin) IgG2a Human ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91117 ¥5,873 Buy Now
10 µg 91118 ¥1,233 Buy Now
AbFlex™ TET1 antibody (rAb) IgG2a Mouse WB 100 µg 91171 ¥5,438 Buy Now
10 µg 91172 ¥1,088 Buy Now